Light-Induced Synthesis of Anthocyanin in Carrot Cells in Suspension

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Abstract
Takeda, J. 1988. Light-induced synthesis of anthocyanin in carrot cells in suspension. I. The factors affecting anthocyanin production.—J. exp. Bot. 39: 1065–1077. A light-triggered anthocyanin-synthesizing system was established for carrot cells in suspension. A few days after transfer of the cells to a 2,4-dichlorophenoxyacetic acid (2,4-D)-free medium in the dark, light irradiation triggered anthocyanin synthesis and concomitantly stopped expansion growth. Over 90% of the cells synthesized anthocyanin without cell division. By lowering the concentration of phosphate or both nitrogen and phosphate and delaying the time of onset of irradiation, the production of anthocyanin per cell increased to a maximum level of 0–8 μmol anthocyanin per 106 cells. A change in the physiological state of cells (light-insensitive to light-sensitive state) induced by the transfer to 2,4-D-free medium is suggested to be a prerequisite for the light-triggered synthesis of anthocyanin.