Glucuronide metabolism in plants. 3. Triterpene glucuronides

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Abstract
Both the glucuronide residues in ammonium glycyrrhizinate were removed by hydrolysis with [beta]-glucuronidase, proving that this glucuronido-glucuronide has the [beta][beta]''-configuration. Although it contains a glucuronide residue, beet saponin was not hydrolyzed by [beta]-glucuronidase. Chro-matographic analysis of an acid hydrolysate revealed the presence of a 2nd sugar, probably glucose, in this glycoside. The possible structure of the glycoside group is discussed. Beet saponin and ammonium glycyrrhizinate acted as non-competitive inhibitors in the hydrolysis of phenolphthalein glucuronide by [beta]-glucuronidase. In addition, the glycyrrhizinate behaved, as expected, as a competing substrate for the enzyme.