AN ANTIGENIC STUDY OF HUMAN-PLASMA CELLS IN NORMAL TISSUE AND IN MYELOMA - IDENTIFICATION OF A NOVEL PLASMA-CELL ASSOCIATED ANTIGEN

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  • 1 July 1986
    • journal article
    • research article
    • Vol. 65  (1) , 112-119
Abstract
A mouse monoclonal antibody named BU11 which detects an antigen strongly expressed on human plasma cells is described. The antibody stains plasma cells in tonsil sections, fresh and cultured plasmacytoid cells from the bone marrow of patients with multiple myeloma and cells of the plasmacytoid cell line RPMI 8226 used as the immunogen. In vitro studies of pokeweek mitogen (PWM) stimulated peripheral blood B cells and Epstein-Barr virus (EBV) stimulates tonsil B cells show that the antigen is present mainly on cells coexpressing the OKT10 antigen and containing cytoplasmic immunoglobulin (cIg). The BU11 antigen is expressed weakly on some normal B cells and is not present on T cells, monocytes or granulocytes. The antigen is of molecular weight 58kD under reducing conditions and is biochemically distinct from previously described plasma cell antigens.