Real‐time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein
Open Access
- 23 April 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 449 (2-3) , 277-283
- https://doi.org/10.1016/s0014-5793(99)00433-0
Abstract
We produced transgenic mouse lines that accumulate mutated green fluorescent protein (EGFP) in sperm acrosome, a membrane limited organelle overlying the nucleus. The sperm showed normal fertilizing ability and the integrity of their acrosome was easily examined in a non‐invasive manner by tracing the GFP in individual ‘live’ sperm with fluorescent microscopy. The time required for the dispersal of acrosomal contents was demonstrated to be approximately 3 s after the onset of acrosome reaction.Keywords
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