Determination of Iron in the Acid-Soluble Fraction of Human Erythrocytes
- 1 January 1972
- journal article
- Published by S. Karger AG in Acta Haematologica
- Vol. 47 (3) , 157-163
- https://doi.org/10.1159/000208510
Abstract
In extracts of the acid-soluble fraction of human erythrocytes, iron exists in form of complexes with nucleotides, and during separation on Dowex-I columns it was completely eluted in that form. The results indicate that about 50% of Fe present in the extracts formed complexes with ATP, and the remaining iron was eluted together with AMP, NADP and ADP. Small amounts of Fe added during the preparation of extracts were found in fractions of eluate containing AMP, NADP and ADP and slightly influenced the content of iron in the complex with ATP.Keywords
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