Immunization of Mice with Dysentery Antigen Administered by Gavage and by Voluntary Drinking.

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Abstract
In the course of attempts to infect white mice by gavage with living Shigella sonnei, immunity developed. A high degree of immunity resulted if the mice were given large numbers of bacteria as antigen; less immunity followed administration of fewer bacteria as antigen. Six doses of 49 billion living S. sonnei administered by gavage, over 4 consecutive days, stimulated complete immunity against intra-perit. injn. of 16 to 160,000 MLD of homologous organisms suspended in sterile mucin. 3-8 daily doses of 1.4 billion living S. sonnei, or 1.1 billion killed S. sonnei, given by gavage, stimulated a significant degree of immunity in the mice when challenged 7 days later with intraperit. injn. of 0.5 to 1280 MLD of the homologous organisms. Three daily doses stimulated sufficient active immunity to protect at least 50% of the mice and 8 daily doses afforded protection to at least 80% of the mice. The titer of the immunity increased with increasing number of daily doses of antigen. There was no significant difference in the immunizing effects of living or killed organisms. Mice which drank killed broth culture of S. sonnei over a period of 21 days were immune against 2 to 2048 MLD of the homologous organisms when injd. intraperit. These mice consumed an average of 7.5 billion killed S. sonnei per day.

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