Cyclin A‐mediated inhibition of intra‐Golgi transport requires p34cdc2
- 28 December 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 336 (3) , 549-554
- https://doi.org/10.1016/0014-5793(93)80874-t
Abstract
An in vitro assay was used to study the role of p34cdc2 in cyclin A-mediated vesicular transport inhibition. It was shown that the S-phase kinase p33cdk2 reduced the effect of cyclin A on transport assays performed with sHeLa cytosol, even though histone kinase was strongly activated. Also, transport with FT210 cytosol (which is temperature-sensitive for p34cdc2) was inhibited by cyclin A only at the permissive temperature. However, the phosphatase inhibitor microcystin inhibited transport without any requirement for p34cdc2 activity. These results show that transport is inhibited by cyclin A via p34cdc2, and also by another kinase, possibly downstream of p34cdc2.Keywords
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