An Improved Method for Purification of Lymphocytes

Open Access
Publisher Website
Google Scholar
Abstract
A method that combines glass-bead column filtration, Ficoll–Hypaque gradient separation, discontinuous sucrose gradient, and drastic reduction of cell transfers is described. The procedure gives a high yield of pure human lymphocytes from small amounts of blood, good preservation of B cell/T cell ratio, and sufficient material for subsequent biochemical studies.