Determination of Folic Acid in Commercial Diets by Anion-Exchange Solid-Phase Extraction and Subsequent Reversed-Phase HPLC

Abstract
Folic acid at the .mu.g/g level is determined in total nutritional diets by concentration on disposable commercial anion exchange columns followed by elution with a concentrated salt solution and subsequent reversed-phase HPLC [high performance liquid chromatography] with absorbance detection at 365 nm. The method is specific for folic acid with respect to some known degradants and electrochemically generated oxidation products.