Expression of Genes in Cloned Murine Cell Lines that can be Maintained in Both Interleukin 2‐ and Interleukin 3‐Dependent Growth States

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Abstract
Two cloned murine cell lines. FD.C/1 and 32Dcl-23 exhibit switching of lymphokine-dependent growth states. The bone marrow-derived FD.C/1 and 32Dcl-23 cell lines are normally grown in culture medium supplemented with inierleukin 3 (IL3). The replacement of IL3 with interleukin 2 (IL2) in the medium results in an increase in IL2 receptor expression in FD.C/1 and 32Dcl-23 cells and the switching of cells lo an IL2-dependent growth state. We have compared patterns of protein and phosphoprotein synthesis, as well as the expression of the c-abl, c-ras, c-myb, and c-fos oncogenes in these cell lines maintained in IL3- and I L2-dependent growth states. The synthesis of a series of proteins and phosphoproteins are identified with each of the lymphokine-dependent growth states. All of the oncogenes examined are expressed in both IL2- and lL3-dependent cells and are not altered by phenotypic changes in lymphokine growth dependence. The relationship of oncogene expression to intracellular pathways regulated by lymphokine-receptor interactions is considered.