Nuclear and cytoplasmic localization of different basic fibroblast growth factor species
- 1 July 1990
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 144 (1) , 108-114
- https://doi.org/10.1002/jcp.1041440114
Abstract
The subcellular distribution of basic fibroblastic growth factor (bFGF) was analyzed by subcellular fractionation and immunofluorescence to gain insight into potential mechanisms for its release from cells. Subcellular fractionation of either SK‐Hep‐1 cells or NIH 3T3 cells transfected with a bFGF cDNA revealed that the 18 kd form of bFGF was found primarily in the cytosolic fraction, whereas the 22 and 24 kd forms of bFGF were found preferentially in ribosomal and nuclear fractions. Analysis of bFGF distribution by immunofluorescence using an antibody that recognized all forms of bFGF indicated both cytoplasmic and nuclear localization but failed to reveal any growth factor in structures representing secretory vesicles. Therefore, bFGF has a distribution inconsistent with that of a secretory protein.This publication has 34 references indexed in Scilit:
- Recent developments in the cell biology of basic fibroblast growth factor.The Journal of cell biology, 1989
- THE HEPARIN-BINDING (FIBROBLAST) GROWTH FACTOR FAMILY OF PROTEINSAnnual Review of Biochemistry, 1989
- An amino-terminally extended and post-translationally modified form of a 25kD basic fibroblast growth factorBiochemical and Biophysical Research Communications, 1989
- Both normal and tumor cells produce basic fibroblast growth factorJournal of Cellular Physiology, 1986
- Postfixation detergent treatment for immunofluorescence suppresses localization of some integral membrane proteins.Journal of Histochemistry & Cytochemistry, 1985
- Post-synthetic modifications of nuclear proteins high mobility group proteins are methylatedBiochemical and Biophysical Research Communications, 1979
- Distribution of NG, NG-dimethylarginine in nuclear protein fractionsBiochemical and Biophysical Research Communications, 1977
- Identification of NG, NG-dimethylarginine in a nuclear protein from the lower eukaryote Physarum polycephalum homologous to the major proteins of mammalian 40S ribonucleoprotein particlesBiochemical and Biophysical Research Communications, 1977
- ISOLATION AND CHARACTERIZATION OF GOLGI MEMBRANES FROM BOVINE LIVERThe Journal of cell biology, 1969
- Enzymatic methylation of protein fractions from calf thymus nucleiBiochemical and Biophysical Research Communications, 1967