Abstract
The LH activity of rat plasma was estimated by the ovarian ascorbic acid depletion method. Significant LH activity was found in plasma from rats which had been ovariectomized for 1-4 months. A single subcutaneous injection of 1.0 μg of estradiol benzoate produced a marked decrease in this activity on assay 3 days later. The LH activity in plasma of such estrogen-treated, ovariectomized rats was significantly elevated within 10 minutes after the intravenous injection of an acidic extract of rat stalk-median eminence tissue, whereas injection of extract from cerebral cortex was without significant effect. It is suggested that stalk-median eminence tissue contains a specific LH-releasing factor.

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