The extravascular pool of albumin in the non-diuretic rat kidney estimated by means of radioactive tracers and quantitative immunochemistry

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Abstract
The extravascular pool of albumin in the non-diuretic rat kidney was determined as the difference between total and intravascular albumin pools. Thetotal pool of albumin was determined in two ways in the same kidney: 1. as the distribution volume of rat endogenous albumin measured immunochemically and 2. as the distribution volume of125I-labelled rat albumin equilibrated for 4 days. The distribution volume of131I human albumin after 1 min equilibration was taken as a measure of theintravascular pool. Total albumin pool estimated as rat endogenous albumin volume of distribution averaged 13.4, 25.1, and 43.0 μl per 100 mg tissue in cortex, outer and inner medulla, respectively. Similar figures were obtained using125I-labelled rat albumin. Intravascular pool of albumin in the 3 zones averaged 7.2, 19.7, and 26.8 μl per 100 mg tissue. The calculated extravascular fraction of albumin averaged 0.44, 0.21, and 0.35 for cortex, outer and inner medulla, respectively.

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