DEVELOPMENT AND CHARACTERIZATION OF ESTABLISHED CELL LINES FROM PRIMARY AND METASTATIC REGIONS OF HUMAN ENDOMETRIAL ADENOCARCINOMA

Google Scholar
  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (6) , 1777-1785
Abstract
Cell lines designated SNG-P and SNG-M were established from operation specimens of primary and metastatic regions of human endometrial adenocarcinoma. The cell lines grew well without interruption for over 13 mo. and were subcultivated > 65 times. They continue to exhibit stable growth. The cultured cells appeared epithelial in shape, showing a pavement arrangement and multilayering without contact inhibition. The cytology revealed anaplastic and pleomorphic features. On EM observation, most of the cultured cells were characterized by indented nuclei with multiple large nucleoli and desmosomal contact. The chromosomal number varied widely and showed aneuploidy, but the modal chromosomal number was stable at the diploid range. No marker chromosome could be identified. Both of these cell lines were transplanted to an immune depressed hamster cheek pouch and produced a tumor resembling the original.