Effect of Intracellular Ca2+ Chelation with the Acetoxymethyl Ester-Derived Form of Bis(o-Aminophenoxy)Ethane-N,N,N,N′,N′-Tetraacetic Acid on Meiotic Division and Chromosomal Segregation in Mouse Oocytes

Abstract
Purpose: Our purpose was to ascertain the effect of intracellular Ca 2+ chelation on the chromosomal distribution and segregation of mouse oocytes during maturation in vitro. Methods: Germinal vesicle oocytes were loaded with the acetoxymethyl ester-derived form of bis(o-aminophenoxy)-ethane-N,N,N′,N′-tetraacetic acid (BAPTA-AM). Chromosomal distribution and segregation of control and BAPTA-AM-treated metaphase II (MII) oocytes were evaluated at 16 hr, and intracellular ATP content at 0, 1, and 16 hr after BAPTA-AM loading. Results: BAPTA-AM treatment decreased (P ≤ 0.05) the potential for in vitro maturation, increased (P ≤ 0.0001) the percentage of oocytes displaying an abnormal distribution of metaphase II chromosomes in the meiosis II spindle and aneuploidy, and decreased (P ≤ 0.005) the ATP content at 0, 1, and 16 hr of culture compared to the control groups. Conclusions: These findings raise some concern about any other condition/drug that may directly or indirectly decrease the intracellular Ca 2+ concentration in human oocytes.

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