HPLC Separation of Flavonoids and Flavonoid Glycosides Using a Polystyrene/Divinylbenzene Column

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Abstract
A polystyrene-divinylbenzene column was investigated for the separation of four different classes of flavonoids and flavonoid glycosides. These analytes were separated using either methanol-water or acetonitrile-water gradients. Acetonitrile-water gradients were judged to be superior for the overall separation of the four classes of flavonoids and flavonoid glycosides. Correlations between chemical structure, and retention and separability are discussed for the various HPLC gradient systems.

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