Spectroscopic evidence for an intramolecular RNA triple helix
- 5 December 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 355 (3) , 297-300
- https://doi.org/10.1016/0014-5793(94)01228-8
Abstract
A 29-base RNA oligomer has been chemically synthesized and shown to form an intramolecular triple helix in solution at acidic pH. Assignment of the majority of the exchangeable proton NMR resonances demonstrated the Watson—Crick and Hoogsteen base pairings consistent with folding to form pyrimidine—purine—pyrimidine base triplets. FTIR spectroscopy provided independent evidence of base triplet formation, and indicated a predominately C3′-endo sugar pucker. UV absorption as a function of temperature suggested monophasic melting behaviour, which was confirmed by NMR of the imino protons.Keywords
This publication has 23 references indexed in Scilit:
- Solvent suppression with symmetrically-shifted pulsesJournal of the American Chemical Society, 1993
- A possible family of B-like triple helix structures: Comparison with the Arnott A-like triple helixBiochemistry, 1993
- Proton nuclear magnetic resonance assignments and structural characterization of an intramolecular DNA triplexJournal of Molecular Biology, 1992
- Structure of G.cntdot.T.cntdot.A triplet in an intramolecular DNA triplexBiochemistry, 1992
- Modelling of the three-dimensional architecture of group I catalytic introns based on comparative sequence analysisJournal of Molecular Biology, 1990
- Phylogenetic and genetic evidence for base-triples in the catalytic domain of group I intronsNature, 1990
- Formation of a stable triplex from a single DNA strandNature, 1990
- RNA fragment r(CGm5CGCG) that exhibits two conformations in slow exchange on the NMR time scale in low salt solutionJournal of the American Chemical Society, 1987
- A two-dimensional nuclear overhauser experiment with pure absorption phase in four quadrantsJournal of Magnetic Resonance (1969), 1982
- FORMATION OF A THREE-STRANDED POLYNUCLEOTIDE MOLECULEJournal of the American Chemical Society, 1957