A cDNA clone closely associated with non-A, non-B hepatitits
- 1 May 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (9) , 2685-2689
- https://doi.org/10.1093/nar/18.9.2685
Abstract
A lambda gtl1 cDNA library was constructed from RNA purified from hepatitis B viral surface antigen-negative human plasma with high alanine aminotransferase activity. A cDNA clone, designated s C8-2, was isolated by immunoscreening with mixed sera from non-A, non-B hepatitis (NANBH) carrier and convalescent chimpanzees. The recombinant protein produced by C8-2 reacted specifically with sera of patients in the chronic phase of NANBH. The sequence of C8-2, 269 bp, did not hybridized with any human or chimpanzee genomic DNA, and had no homology with those of primates and viruses. The existence of this sequence in RNA of possibly infectious plasma was shown by RNA blot hybridization and by Southern blot analysis of products amplified by the polymerase chain reaction. These results strongly suggest that C8-2 is derived from the agent of this viral hepatitis.Keywords
This publication has 13 references indexed in Scilit:
- Isolation of a cDNA cLone Derived from a Blood-Borne Non-A, Non-B Viral Hepatitis GenomeScience, 1989
- An Assay for Circulating Antibodies to a Major Etiologic Virus of Human Non-A, Non-B HepatitisScience, 1989
- Polymerase chain reaction amplification of rhinovirus nucleic acids from clinical materialNucleic Acids Research, 1988
- [7] λgt 11: Gene isolation with antibody probes and other applicationsPublished by Elsevier ,1987
- Production of antibody associated with non-A, non-B hepatitis in a chimpanzee lymphoblastoid cell line established by in vitro transformation with Epstein-Barr virus.Proceedings of the National Academy of Sciences, 1985
- ISOLATION OF A VIRUS FROM CHIMPANZEE LIVER CELL CULTURES INOCULATED WITH SERA CONTAINING THE AGENT OF NON-A, NON-B HEPATITISThe Lancet, 1984
- Prediction of protein antigenic determinants from amino acid sequences.Proceedings of the National Academy of Sciences, 1981
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Non-A, Non-B Hepatitis: Ultrastructural Evidence for Two Agents in Experimentally Infected ChimpanzeesScience, 1979
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977