Environmental rRNA inventories miss over half of protistan diversity
Open Access
- 16 December 2008
- journal article
- Published by Springer Nature in BMC Microbiology
- Vol. 8 (1) , 222
- https://doi.org/10.1186/1471-2180-8-222
Abstract
The main tool to discover novel microbial eukaryotes is the rRNA approach. This approach has important biases, including PCR discrimination against certain rRNA gene species, which makes molecular inventories skewed relative to the source communities. The degree of this bias has not been quantified, and it remains unclear whether species missed from clone libraries could be recovered by increasing sequencing efforts, or whether they cannot be detected in principle. Here we attempt to discriminate between these possibilities by statistically analysing four protistan inventories obtained using different general eukaryotic PCR primers.Keywords
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