Induction of Cell-mediated Cytotoxic Immunity to Sperm-specific Lactate Dehydrogenase-C4 in SJL/J Female Mice 1

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Abstract
SJL/J female mice were tested for a cell-mediated cytotoxic response to sperm-specific lactate dehydrogenase C4 (LDH-C4). This response was demonstrated with a 51Cr release assay. Mouse LDH-C4 was coupled to EL-4 tumor cells. These cells were then labeled with 51Cr and mixed with splenocytes from LDH-C4-immune and -nonimmune mice. Specific lysis of the tumor cells by splenocytes from LDH-C4-immune mice was detected at 7 days and 14 days following a single footpad immunization. Since LDH-C4 is present on the surface of sperm, these results support the suggestion that cytotoxic removal of sperm from the female reproductive tract is 1 of the mechanisms whereby fertility is reduced following immunization with this enzyme.

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