Two proteins regulate the cell wall extensibility and the yield threshold in glycerinated hollow cylinders of cowpea hypocotyl

Publisher Website
Google Scholar
Abstract
Glycerinated hollow cylinders of hypocotyl segments excised from the elongation region of cowpea seedlings were heated for 15s in 50% glycerol at 70, 80 or 90°C. Their in vitro yield threshold tension (y) and extensibility (φ) were determined by stress‐strain experiments under the perfusion of solutions of pH 4·0 or 6·2. The decrement in y and the increment in φ with acidification were extinguished at 80 and 90°C, respectively. Moreover, such changes in φ and y with acidification were prevented by proteinase treatment for 6 and 10 h, respectively. These results suggest that these two cell wall mechanical properties are controlled, respectively, by two functional proteins activated by acid.