2,2'-DIHYDROXY-3,3'-DI-TERT-BUTYL-5,5'-DIMETHOXYDIPHENYL, A NEW METABOLITE OF 2-TERT-BUTYL-4-METHOXYPHENOL IN THE RAT

  • 1 January 1983
    • journal article
    • research article
    • Vol. 11  (6) , 581-584
Abstract
A method was developed to determine 2-tert-butyl-4-methoxyphenol (BHA) and its metabolite di-BHA in rat plasma and tissues using gas chromatography-mass spectrometry with selected ion detection. Deuterium labeled BHA-d3 and di-BHA-d6 were synthesized and added to the tissue specimens as internal standards before methylene chloride extraction. The extracted compounds were derivatized with trifluoroacetic anhydride and analyzed by selected ion monitoring. Rat plasma and intestine concentrations of BHA and di-BHA at different times (0.15-24 h) folllowing the oral administration of a single dose of BHA (2 g/kg body wt) were determined. BHA and di-BHA were present in all samples, their concentration peaking with 1 h after treatment. While in the intestine BHA levels were .apprx. 10-fold higher than those of di-BHA, in the plasma they were 100-15-fold higher. Rat intestine transformed in vivo BHA into di-BHA and this organ was probably the major site where this transformation occurred.

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