Nucleotide sequence of the SUF2 frameshift suppressor gene of Saccharomyces cerevisiae.

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Abstract
To elucidate the molecular mechanism of frameshift suppression by the SUF2 gene of yeast, the sequences of DNA fragments carrying the SUF2-1 and suf2+ alleles of the gene and surrounding regions were determined. Comparison of the suppressor and wild-type sequences indicates that the SUF2 gene product is a proline tRNA. Disregarding possible base modifications, the wild-type suf2+ anticodon of the tRNA inferred from the DNA sequence is 3''-GGA-5''. The SUF2-1 mutation represents the insertion of a G .cntdot. C base pair at a position in the gene that corresponds to the anticodon loop of the tRNA. Replacement of the wild-type suf2+ anticodon by 3''-GGGA-5'' 4-base anticodon enables the SUF2-1 tRNA to suppress the 5''-CCCU-3'' 4-base codons generated as the result of the his4-712 and his4-713 frameshift mutations. This nontriplet codon-anticodon interaction restores the correct reading frame and allows synthesis of a functional his4 protein.