ELISA detection of fentanyl in horse urine and plasma

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Abstract
The prototype of a commerical ELISA test kit designed for fentanyl determination in human urine has been evaluated for screening fentanyl in horse urine and plasma. The measurement of fentanyl after intravenous (2 mg) and intramuscular (0.25 mg) administration in undiluted plasma was not reproducible while accurate quantification of fentanyl in urine greatly depends on the composition of the horse urine. The ELISA assay, however, is simple and could be successfuly used for quantitative measurements in diluted urine and for rapid qualitative screenign for fentanyl in large numbers of urine samples.

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