Determination of selenium in blood serum by inductively coupled plasma atomic emission spectrometry with pneumatic nebulization

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Abstract
The possibility of determining selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW=6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160 °C was sufficient to decrease the carbon content in the serum sample to 5%–10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01–0.02 mg/L and the limit of quantification (RSD 10%) is 0.03–0.07 mg/L using Se I 196.090 nm line at an integration time of 10–2 s. The method was tested by analysis of porcine blood serum and the serum reference material Seronorm MI 0181.

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