Zur Trennung D-Peptid spaltender Enzyme

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Abstract
Two preparations that cleave D-peptides were obtained from human kidney by treatment with aluminium oxide gel and fractionation on Sephadex. The preparation designated "Gly-D-Leu-cleaving activity" was separated into 2 fractions by gel filtration on Sephadex G-200, and the relationship of these to the known Gly-D-Leu-cleaving enzymes is discussed. From the gel filtration properties of the fractions it is concluded that the fraction eluted before leucine aminopeptidase does not correspond to the known purified Gly-D-Leu-cleaving preparations of pig kidney. Under standard conditions, the isolated Gly-D-Leu-cleaving activity does not attack D-Leu-Gly. The kidney preparation designated "D-Leu-Gly-cleaving activity" is different from the described "Gly-D-Leu-cleaving activity". Its distribution in various human organs is described. The 2 preparations have the same activity towards the corresponding L-peptides. The possible existence of independent L-, D-, and dehydro-peptidases is discussed briefly.

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