Status of Endogenous Avian RNA Tumor Virus in Marek's Disease Lymphoblastoid Cell Lines and Susceptibility of Those Lines to Exogenous RNA Tumor Viruses

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Abstract
Three lymphoblastoid cell lines from Marek''s disease (MD) tumors, 2 MD virus (MDV) producer lines (MSB-1 and HPRS-1) and a nonproducer line (RPL-1) were studied for the expression of avian leukosis sarcoma (ALS) viruses. The MSB-1 line was free of all known endogenous expressions, including replicating virus (RAV[Rous-associated virus]-0), group-specific (gs) antigens and chick helper factor (chf). The RPL-1 and HPRS-1 were positive for gs antigens and chf. The RPL-1 and MSB-1 lines showed no evidence of an exogenous DNA provirus by nucleic acid hybridization (HPRS-1 line was not tested for that DNA). All 3 lymphoblastoid cell lines were susceptible to exogenous infection with both sarcoma and leukosis viruses of subgroup A but varied in susceptibility to subgroups B and C. All were resistant to subgroups D and E.