A Biopsy Procedure for the Nondestructive Determination of Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) Infection inPenaeus vannamei

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Abstract
The shrimp Penaeus vannamei is frequently an asymptomatic carrier of infectious hypodermal and hematopoietic necrosis virus (IHHNV). In addition, there is increasing evidence that P. vannamei may also suffer severe disease from infection by IHHNV under some culture conditions. Hence, a nondestructive technique has been developed that has the potential to replace the old method of destructive subsampling, at least with this penaeid species. The new procedure is simpler, faster, less labor intensive, and equally precise when compared with the old method. The procedure involves the excision of the first pereiopod, followed by standard histological examination of the appendage nerve cord for the presence of pathognomonic Cowdry type A intranuclear inclusions. The nondestructive nature of this new technique may be its most important asset; animals tested for IHHNV via this method need not be sacrificed and are thus still available for use as brood stock.

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