Combination of Certain Fluorane Derivative dyes with Bacterial cells at Different Hydrogen on Concentrations

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Abstract
Quantitative detns. of the amt. of dye combining with cells of 5 common bacterial spp. at different pH levels have been made using 4 fluorane derivatives, eosin B, eosin Y, erythrosin B, and rose Bengal. Rose Bengal, the least acid of these dyes, combines most readily with the cells over the range pH 7 to pH 3. Adsorption of the dyes in this series was in general inversely related to the degree of acidic ionization of the dye molecule. All dyes combined with the cells more readily at the lower pH levels. A brief discussion of the theoretical implications in staining is given.