Assignment of acetyl groups to O‐2 and/or O‐3 of pectic oligogalacturonides using negative electrospray ionization ion trap mass spectrometry
- 6 June 2003
- journal article
- research article
- Published by Wiley in Journal of Mass Spectrometry
- Vol. 38 (6) , 641-648
- https://doi.org/10.1002/jms.478
Abstract
Partially acetylated and methylated oligogalacturonides produced by enzymatic hydrolysis of sugar beet pectin were analysed by negative electrospray ionization ion trap mass spectrometry (ESI‐ITMS). The 18O labelling of the oligomer reducing end allowed the precise assignment of the fragments resulting from glycosidic bond and cross‐ring cleavages. The collisional‐induced dissociation of the Ci and Zj fragment ions through sequential MSn experiments always displayed 0, 2A‐type cross‐ring cleavage ions which were related to C2H4O2 losses. These 0, 2A ions appeared to be highly diagnostic ions allowing the precise location of the acetyl groups to the O‐2 and/or O‐3 of the acetylated galacturonic acid residues. Copyright © 2003 John Wiley & Sons, Ltd.Keywords
Funding Information
- European Community (Europectin QLK3-1999-00089.)
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