Comparative Coliform Densities in Water by Membrane Filter Test and by Multiple Tube Technic

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Abstract
The results of these experiments seem to indicate that neither the membrane filter nor the mechanics of filtration are inhibitive to coliform bacteria. The discrepancies between the membrane filter and the tube technique appear to increase with the time and distance from fresh pollution, and the difficulty of recovery increases with the ratio of noncoliform to coliform. Studies of several media indicate that none of those now in use are completely satisfactory for the total recovery of coliforms in all waters. The defects lie in failure to permit growth and failure to permit all colonies to exhibit the characteristic sheen now required at any one period of incubation. The most urgent need in connection with the membrane filter seems to be the formulation of a more satisfactory medium. Further work with pure cultures of the coliform group would seem to be an essential part of the approach to this problem.

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