Effects of segregation and selection on instability of plasmid pACYC184 in Escherichia coli B
Open Access
- 1 November 1987
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 169 (11) , 5314-5316
- https://doi.org/10.1128/jb.169.11.5314-5316.1987
Abstract
We use a mathematical model to analyze the dynamics of loss of nonconjugative pACYC184 from populations of Escherichia coli B in glucose-limited continuous culture. This model incorporates both plasmid segregation and selection against plasmid carriage. It is concluded that there is intense selection against plasmid carriage (s = 0.3 per culture generation), which amplifies the frequency of segregants arising de novo.This publication has 33 references indexed in Scilit:
- The insertion of large pieces of foreign genetic material reduces the stability of bacterial plasmidsPlasmid, 1986
- Effects of recombinant plasmid content on growth properties and cloned gene product formation in Escherichia coliBiotechnology & Bioengineering, 1985
- A review of mini-F plasmid maintenancePlasmid, 1985
- Expression of tetracycline resistance in pBR322 derivatives reduces the reproductive fitness of plasmid-containing Escherichia coliGene, 1985
- Kinetic study of instability of recombinant plasmid pPLc23trpAl in E. coli using two‐stage continuous culture systemBiotechnology & Bioengineering, 1985
- Structural and functional analysis of the par region of the pSC101 plasmidCell, 1984
- The Maintenance of Plasmid-containing Organisms in Populations of Escherichia coliMicrobiology, 1981
- Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritanceCell, 1980
- Generation time-prolonging R plasmids: Correlation between increases in the generation time of Escherichia coli caused by R plasmids and their molecular sizePlasmid, 1980
- The Influence of the Growth Environment on the Stability of a Drug Resistance Plasmid in Escherichia coli K12Journal of General Microbiology, 1979