Regulation of lipoprotein lipase synthesis in 3T3-L1 adipocytes by cachectin Further proof for identity with tumour necrosis factor
- 1 December 1986
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 240 (2) , 601-604
- https://doi.org/10.1042/bj2400601
Abstract
We investigated the mechanism by which the endotoxin-induced macrophage secretory protein cachectin is able to suppress the activity of lipoprotein lipase in 3T3-L1 adipocytes. The loss in activity results from an effect on the synthesis of the enzyme, as determined by a decreased incorporation of [35S]methionine into immunoprecipitable lipoprotein lipase. The results were nearly identical whether crude conditioned medium or a highly purified preparation was utilized as a source of cachectin. [35S]Methionine incorporation into acid-precipitable protein was minimally affected by purified cachectin, suggesting that the suppression of the lipoprotein lipase was not due to a general suppression of protein synthesis. These results, taken together with our previous work, provide additional evidence that cachectin and tumour necrosis factor are functionally identical.This publication has 18 references indexed in Scilit:
- Production and characterization of monoclonal antibodies against recombinant human tumor necrosis factor/cachectinBiochemical and Biophysical Research Communications, 1986
- Selective inhibition of synthesis of enzymes for de novo fatty acid biosynthesis by an endotoxin-induced mediator from exudate cells.Proceedings of the National Academy of Sciences, 1983
- Lipoprotein lipase suppression in 3T3-L1 cells by an endotoxin-induced mediator from exudate cells.Proceedings of the National Academy of Sciences, 1982
- Insulin regulation of lipoprotein lipase activity and release in 3T3-L1 adipocytes. Separation and dependence of hormonal effects on hexose metabolism and synthesis of RNA and protein.Journal of Biological Chemistry, 1979
- Fluorographic detection of radioactivity in polyacrylamide gols with the water-soluble fluor, sodium salicylateAnalytical Biochemistry, 1979
- Post-translational regulation of lipoprotein lipase activity in adipose tissueBiochemical Journal, 1978
- Development of hormone receptors and hormonal responsiveness in vitro. Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells.Journal of Biological Chemistry, 1978
- Studies of lipoprotein lipase during the adipose conversion of 3T3 cellsCell, 1978
- Induction of lipogenesis during differentiation in a "preadipocyte" cell line.Journal of Biological Chemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970