A New Methylation Method for the Gas Chromatography of Barbituric Acids

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Abstract
A methylation method has been developed, which consists in refluxing an acetone solution of free barbituric acids and of methyl iodide in the presence of solid potassium carbonate as condensating agent. The solution is then injected directly into the gas chromatograph without further extraction or concentration steps. This technique is shown to be suitable for the quantitative determination of barbituric acids in the microgram and nanogram range. All reactants are non hazardous and therefore can be used in day to day laboratory work.