Radioimmunoassay of Plasma Pregnenolone-Sulfate

Publisher Website
Google Scholar
Abstract
This report describes a radioimmunoassay method for the direct measurement of pregnenolone-sulfate (5Δ-P-S) in diluted plasma. Utilizing a specific anti-5Δ-P-S antiserum which reacted both with pregneolone (5Δ-P) and its sulfate (5Δ-P-S), we have measured 5Δ-P-S with accuracy in small aliquots of diluted plasma. Because the plasma concentration of 5Δ-P-S is relatively high, the effect of 5Δ-P and other steroids on the assay was negligible. The usable range of the standard curve was between 0.1 and 25 ng of 5Δ-P-S. The range of values obtained by this method for plasma 5Δ-P-S in peripheral maternal plasma and in the newborn compared favorably with the range of levels observed with other reliable but more cumbersome methods.

This publication has 7 references indexed in Scilit: