HindIII and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA

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Abstract
The DNA of 2 closely related orthopoxviruses, rabbit poxvirus (RPV) and vaccinia virus (VV), were mapped by overlapping-fragment analysis using restriction endonucleases HindIII and Sst I. The exact arrangement of these fragments was accomplished by total digestion of isolated partial restriction products and by end-fragment determination. RPV and VV DNA showed identical restriction patterns in an internal region comprising about 60% of the genome. The size, by electrophoretical analysis of the RPV DNA, was 118 .times. 106 daltons, some 6 .times. 106 daltons less than VV DNA. The 2 opposite terminal restriction fragments of RPV DNA cross-hybridized to each other.