D-GLUCOSE ANOMERIC PREFERENCE OF HEXOKINASES IN HIGHER ANIMALS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 84  (4) , 993-995
Abstract
The D-glucose anomeric preference of hexokinases isolated from rat liver, brain and skeletal muscle, and bovine retina was studied using the glucose-6-phosphate dehydrogenase-NADP system. The ratios of maximum phosphorylation rates of .beta.-D-glucose to those of .alpha.-D-glucose were 1.33, 1.46 and 1.54 for hexokinase types I, II and III from rat liver, 1.45 and 1.63 for type I from rat brain and bovine retina, 1.53 for type II from rat skeletal muscle, and 0.55 (when determined at 5 mM) for type IV (glucokinase) from rat liver, respectively.

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