Brain tubutin and actin cDNA sequence: isolation of recombinant plasmids
- 1 January 1980
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (16) , 3553-3564
- https://doi.org/10.1093/nar/8.16.3553
Abstract
Rat brain mRNA enriched for tubulin and actin sequences was used to prepare double stranded cDNA. A library of recombinant clones was constructed by inserting the dsDNA into the Pst1 site of pBR322 plasmid and transformation of E. coli chi 1776 host. Clones bearing sequences coding for tubulin and actin were identified and characterized.Keywords
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