The Existence of a Long-Chain Acyl-CoA Synthetase in Homogenates of Human Blood Platelets
- 1 January 1971
- journal article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 28 (3) , 261-265
- https://doi.org/10.3109/00365517109095698
Abstract
The presence of a long-chain acyl-CoA synthetase in human platelets is demonstrated. The activity was reproducibly assayed in small samples by the formation of acyl-carnitine from fatty acids, CoA, ATP, and carnitine in the presence of carnitine palmityltransferase. The activity increased with increasing carbon number from C10 to C16, suggesting only one long-chain acyl-CoA synthetase in human platelets. The enzyme is probably localized within the cell membrane as the enzyme was latent in intact platelets. The activity in 22 fasting, healthy individuals was 8 to 23 nmol/min/109 platelets, or about 0.5 to 1.5 U/ml packed cells. The activity is compatible with the activity found in several organs from the rat, and with reported rates of fatty acid incorporation into platelet lipids.Keywords
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