PRODUCTION OF MONOCLONAL-ANTIBODY ALPHA-PRO3 RECOGNIZING A HUMAN PROSTATIC-CARCINOMA ANTIGEN

  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (4) , 1215-1222
Abstract
The monoclonal antibody .alpha.Pro3 recognizes an antigen concentrated in human primary prostatic carcinoma tissue removed surgically. Although the antigen was detectable in extracts of human normal and malignant nonprostatic tissue as well as in benign prostate tissue, quantitative absorption analysis revealed a substantially greater quantity of the antigen in malignant prostate tissue. The antigen recognized by .alpha.Pro3 in primary prostatic carcinoma has an apparent nonreduced MW of 175,000 and an apparent subunit MW of 54,000. This antigen, p54, appears to be present on the surface of cultured prostatic tumor cells of the PC-3 cell line, but its location in vivo has not been defined. Successful competition between .alpha.Pro3 and prostatic carcinoma patient serum Ig for a MW 54,000 antigen (reduced MW) present in prostatic carcinoma tissue extract suggests that p54 may play a significant role in the immunobiology of prostatic carcinoma. .alpha.Pro3 has potential as a sensitive probe for an antigen relevant to human tumor biology.

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