Phospholipase A of Guinea Pig Spermatozoa: Its Preliminary Characterization and Possible Involvement in the Acrosome Reaction. (guinea pig/spermatozoa/acrosome reaction/phospholipase A)

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Abstract
Phospholipase A activity was demonstrated in guinea pig spermatozoa using [U[uridine]-14C] phosphatidyl choline as a substrate. The activity had a neutral pH optimum, was stimulated by Ca2+ and low concentrations of detergent and was inhibited by EDTA, mepacrine and p-bromophenacyl bromide. Appropriate concentrations of mepacrine and p-bromophenacyl bromide inhibited the acrosome reactions of capacitated spermatozoa without interfering with their motility. Phospholipase A apparently is involved in the acrosome reaction of mammalian spermatozoa.