PROLONGED SURVIVAL OF RAT ORTHOTOPIC LIVER ALLOGRAFTS AFTER INTRATHYMIC INOCULATION OF DONOR-STRAIN CELLS

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Abstract
Permanent donor-specific tolerance to tissue or organ allografts can be readily achieved without immuno-suppression by administration of donor lymphohemato-poietic cells to neonatal rodents. In adult recipients, however, induction of transplantation tolerance by this strategy generally requires intensive cytoablative con ditioning of the recipient. We have now demonstrated that intrathymic inoculation of donor bone marrow or hepatic cells in conjunction with a single dose of antilym-phocyte serum is effective in prolonging survival of DA rat orthotopic liver allografts in LEW strain recipients, which ordinarily rapidly reject such transplants. The unresponsive state achieved is donor-specific, as evidenced by the failure of intrathymic inocula of third-party WF cells to promote survival of LEW recipients of orthotopic DA liver allografts. Moreover, intravenous administration of the donor cells fails to extend liver allograft survival, demonstrating that the inoculum must be present in the thymus to promote unresponsive-ness.

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