Isolation and Regulation of Sinorhizobium meliloti 1021 Loci Induced by Oxygen Limitation
Open Access
- 1 August 2001
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (8) , 3728-3731
- https://doi.org/10.1128/aem.67.8.3728-3731.2001
Abstract
Eleven Sinorhizobium meliloti 1021 loci whose expression was induced under low oxygen concentrations were identified in a collection of 5,000 strains carrying Tn 5 - 1063 ( luxAB ) transcriptional reporter gene fusions. The 11 Tn 5 - 1063 -tagged loci were cloned and characterized. The dependence of the expression of the tagged loci on the FixL/FixJ oxygen-sensing two-component regulatory system was examined. Three of the loci were found to be dependent upon fixL and fixJ for their expression, while one locus showed a partial dependence. The remaining seven loci showed fixL - and fixJ -independent induction of expression in response to oxygen limitation. This suggests that in S. meliloti , additional regulatory system(s) exist that respond either directly or indirectly to oxygen limitation conditions.Keywords
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