An Improved Assay for Adenosine in Rat Brain Microdialysates Using Microbore High Performance Liquid Chromatography

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Abstract
A high-performance liquid chromatographic method using a microbore system consisting of a microbore column (100 mm – 1mm I. D.- 3 μm), microbore pump (60 μl/min) and UV absorbance detector has been developed for the determination of adenosine in rat brain microdialysis samples. UV absorbance of adenosine at 259 nm was used to measure adenosine. The limit of detection (LOD) was 50 pg and the limit of quantitation (LOQ) was 10 ng/ml. The methodology is applicable for the quantitation of adenosine in small volumes (3 – 10 (μl) of microdialysate and does not require sample preparation.