CMP–3‐Deoxy‐d‐Glycerol‐d‐Galacto‐Nonulosonic Acid (CMP‐Kdn) Synthetase

1 March 1996

journal article
research article
Published by Wiley in European Journal of Biochemistry

Vol. 236 (3) , 852-855
https://doi.org/10.1111/j.1432-1033.1996.00852.x

Abstract

In this report we present kinetic data of the activation reaction of several synthetic 3-deoxy-D-glycero-D-galacto-nonulosonic acid (Kdn) and N-acetylneuraminic acid (Neu5Ac) analogues catalyzed by the rainbow trout testis CMP-Kdn synthetase. This enzyme showed broad substrate specificity in terms of substitutions at C4 or C5 position of Kdn and Neu5Ac. In contrast, calf brain CMP-N-acylneuraminic acid synthetase had narrow substrate specificity, being active only on various N-acyl analogues of Neu5Ac and only slightly active on Kdn derivatives. Usefulness of the trout testis enzyme for synthesis of various CMP-sialate analogues, which could be donor substrates for sialyltransferases, was demonstrated.

Keywords

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