Abstract
Plasmids that replicate only by means of the cloned Escherichia coli replication origin (orlC) are called mlnichromosomes or oriC-plasalds. In this paper It Is shown that sequences located between oriC and asnA are Involved In maintenance and Incompatibility of mlnlchromosomes. These sequences Include part of the 16kD and 17kD genes, previously allocated within this region (1.2).