NUCLEAR MEMBRANES OF CULTURED MAMMALIAN CELLS IN THE PERIOD PRECEDING DNA SYNTHESIS
Open Access
- 1 April 1965
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 25 (1) , 103-107
- https://doi.org/10.1083/jcb.25.1.103
Abstract
When kidney cells are cultured directly from the rabbit, the nuclear membranes undergo a change that can be measured as an increase in electrophoretic mobility. The change appears to begin immediately upon culture and is maximal 2 hours later, after which the mobility remains constant at the elevated level. Actinomycin D and p-fluorophenylalanine, but not EDTA or ionizing radiation, suppress the increase in nuclear electro-phoretic mobility. With synchronously growing L cells, no change was detected in nuclei from cells taken during various parts of the division cycle.Keywords
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