Circular Dichroism of the Disulfide Groups of Ribonuclease in 6 M Guanidine Hydrochloride
- 1 March 1974
- journal article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 75 (3) , 441-446
- https://doi.org/10.1093/oxfordjournals.jbchem.a130412
Abstract
The contribution of disulfide groups to the observed circular dichroic spectrum of bovine pancreatic ribonuclease [EC 2.7.7.16] in 6M guanidine hydrochloride, pH 3.0, in the near ultraviolet region was estimated by measuring circular dichroic spectra before and after reduction of the groups with dithioerythritol and taking the difference. The groups were found to have a negative circular dichroic band at 262 nm with a residue molar ellipticity of −1,900 deg.cm2-decimole−1 at 5°C. The optical activity was shown to be similar but not identical to the circular dichroic spectrum of N, N'-diacetyl-L-cystine bismethylamide, which is thought to be a good model compound for L-cystine residue.This publication has 0 references indexed in Scilit: