Continuous Separation of Proteins in Electrical Split-Flow Thin (SPLITT) Cell with Equilibrium Operation
- 1 November 1989
- journal article
- research article
- Published by Taylor & Francis in Separation Science and Technology
- Vol. 24 (14) , 1245-1259
- https://doi.org/10.1080/01496398908049900
Abstract
The continuous separation of charged species in an electrical split-flow thin (SPLITT) cell is described. By operating the electrical SPLITT system in an equilibrium mode at a solution pH lying between the isoelectric points of two proteins, a mechanism is available for the rapid and complete separation of the proteins. The theoretical conditions necessary for such a separation are established. The apparatus constructed to test this concept is described. Preliminary experimental results are reported for several proteins, and the complete resolution of a binary mixture of ferritin and cytochrome C is demonstrated.Keywords
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