IDENTIFICATION OF 7-CARBOXYMETHYLGUANINE IN DNA FROM PANCREATIC ACINAR-CELLS EXPOSED TO AZASERINE
- 1 January 1982
- journal article
- research article
- Vol. 42 (4) , 1286-1288
Abstract
Studies were undertaken to determine the identity of an azaserine:DNA adduct. The most probable adduct, 7-carboxymethylguanine, was synthesized. DNA isolated from pancreatic acinar cells treated in culture with [14C]azaserine was hydrolyzed under neutral conditions to liberate N-alkylated purines. The neutral hydrolysate was subjected to high-performance liquid chromatography along with the synthetic standard. One of the radioactive peaks from the treated DNA cochromatographed with 7-carboxymethylguanine in 3 systems: reverse phase, anion exchange and ion pair reverse phase. Azaserine metabolism in acinar cells apparently results in carboxymethylation of DNA, supporting previously proposed models of azaserine degradation.This publication has 8 references indexed in Scilit:
- Studies of pancreatic nodule induction and DNA damage by D-azaserine☆Cancer Letters, 1981
- Studies of DNA damage in rat pancreas and liver by 6-diazo-5-oxo-L-norleucine, ethyl diazoacetate and azaserine☆Cancer Letters, 1981
- Methylation of hamster DNA by the carcinogen N-nitroso-bis (2-oxopropyl)amineCancer Letters, 1981
- New synthesis of azaserineThe Journal of Organic Chemistry, 1978
- DNA-REPAIR IN PRIMARY CULTURES OF RAT HEPATOCYTES1978
- DNA DAMAGE AND REPAIR IN RAT TISSUES FOLLOWING ADMINISTRATION OF AZASERINE1977
- ENZYMATIC DEGRADATION OF AZASERINE1962
- PYRIDOXAL AND METAL ION CATALYSIS OF ALPHA,BETA-ELIMINATION REACTIONS OF SERINE-3-PHOSPHATE AND RELATED COMPOUNDS1957